Hepcidin-24 (human) sequence contains three Cys-Cys fragments synthesized by peptide bond of amide bond, which is very unstable in the production process. For this kind of difficult peptide sequence, it is necessary to design a reasonable production process. Generally speaking, a reasonable condensation agent system can ensure the stable formation of peptide bonds without side reactions; and in the cracking process, antioxidant needs to be added; when air oxidized forms the crude product of the target product, it is recommended to separate and purify in the acid system to ensure the formation of two peptide bond of disulfide bonds is not affected.