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CAS NO:
6769-80-8
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Basic Info
CAS Number
6769-80-8
Product Keywords
5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt,,Bio Products,Reagent,Biochemistry
Is Manufacturer
No
Product Description
Name(En): 5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt
Product Name: 5-溴-6-氯-3-吲哚磷酸对甲苯胺盐
Alternative name：
CAS: 6769-80-8
Product No: D0029
Formula: C15H15BrClN2O4P
Weight: 433.621
Hacceptors: 5
Hdonors: 3
WeightF
AI Product Description
*The following content is generated by AI and is for reference only.
5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt, widely recognized by its CAS number 104978-68-1, is a specialized chemical reagent primarily utilized in biochemical research and diagnostic applications. Its molecular formula is C20H16BrClN2O4P, reflecting a complex structure that combines an indole-based substrate with a phosphate group and a p-toluidine counterion. This compound serves as a critical chromogenic or fluorogenic substrate for the detection of alkaline phosphatase (ALP) activity in various biological systems.
In laboratory settings, this reagent is frequently employed in enzyme-linked immunosorbent assays (ELISA), Western blotting, and histochemical staining protocols. Upon enzymatic hydrolysis by alkaline phosphatase, the phosphate moiety is cleaved from the indolyl ring, generating an unstable intermediate that spontaneously dimerizes to form a highly colored precipitate. The resulting product typically exhibits a deep blue or purple hue, providing high contrast against biological backgrounds for sensitive visualization. The inclusion of the p-toluidine salt enhances the solubility and stability of the substrate in aqueous buffers, ensuring consistent performance during quantitative analysis.
The high sensitivity of this substrate allows researchers to detect minute quantities of target proteins or nucleic acids, making it indispensable in molecular biology and clinical diagnostics. It is particularly valued for its ability to produce insoluble deposits at the site of enzyme activity, which facilitates precise localization within tissue sections without requiring extensive washing steps. Furthermore, the reaction kinetics are well-characterized, enabling reliable quantification of ALP levels in serum, cell lysates, and purified protein samples. Safety protocols must be strictly followed when handling this chemical due to potential irritant properties associated with brominated and chlorinated aromatic compounds. Proper storage in cool, dry conditions away from light preserves its integrity. As a versatile tool, 5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt continues to support advancements in understanding cellular mechanisms and developing novel diagnostic tests across diverse scientific disciplines.
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