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CAS NO:
6976-37-0
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Basic Info
CAS Number
6976-37-0
Product Keywords
Bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane chemical reagent,,Bio Products,Reagent,Chemical Reagent
Is Manufacturer
No
Product Description
Name(En): Bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane
Product Name:(BIS-TRIS)
Alternative name： Bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane
CAS: 6976-37-0
Product No: S0019
Formula: C8H19NO5
Weight: 209.2402
Hacceptors: 6
H
AI Product Description
*The following content is generated by AI and is for reference only.
Bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane, commonly known as Bis-Tris or a derivative of Tris buffer systems, is a sophisticated organic compound widely utilized in biochemical and pharmaceutical research. Its chemical structure features a central nitrogen atom bonded to three hydroxymethyl groups and two 2-hydroxyethyl chains, creating a highly versatile zwitterionic character that facilitates stable buffering across a broad pH range. The molecular formula for this specific reagent is typically represented as C9H19NO5, although variations may occur depending on specific hydration states or salt forms used in commercial preparations. While standard Tris buffers are more ubiquitous, this bis-substituted variant offers enhanced solubility and distinct pKa properties, making it ideal for applications requiring precise pH control in complex biological environments.
The primary application of this reagent lies in protein purification, electrophoresis, and enzyme kinetics studies. Researchers frequently employ it as a buffer component to maintain physiological pH levels during cell lysis and chromatography processes, ensuring the stability and activity of sensitive biomolecules. Unlike simple amines, the multiple hydroxyl groups in its structure allow for effective hydrogen bonding interactions, which can influence protein folding and crystallization outcomes. Furthermore, its low toxicity profile relative to other amine-based buffers makes it suitable for use in mammalian cell culture media and diagnostic assays where cell viability is paramount. In industrial settings, it serves as a key intermediate in the synthesis of specialized chelating agents and surfactants.
Safety protocols dictate handling this powder with standard laboratory precautions, including the use of gloves and eye protection, as inhalation of dust may cause respiratory irritation. Storage should be conducted in a cool, dry place away from strong oxidizing agents to prevent decomposition. Overall, Bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane represents a critical tool in modern molecular biology, bridging the gap between fundamental chemical buffering needs and advanced biotechnological requirements. Its unique structural configuration ensures reliability in experiments demanding high precision, thereby supporting breakthroughs in drug development and genetic engineering. As research methodologies evolve, such specialized reagents continue to play an indispensable role in advancing scientific understanding of cellular mechanisms.
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