Product Description
Name(En): 3-Aminophthalhydrazide
Product Name: 鲁米诺
Alternative name: 3-氨基邻苯二甲酰肼,发光氨
CAS: 521-31-3
Product No: L0031
Formula: C8H7N3O2
Weight: 177.1601
Hacceptors: 5
Hdonors: 3
WeightFrag: 177.162
TDF: 0
Clogp:
AI Product Description
*The following content is generated by AI and is for reference only.
3-Aminophthalhydrazide, commonly known as Luminol, is a pivotal chemical reagent widely utilized in forensic science, analytical chemistry, and biomedical research. Its molecular formula is C8H7N3O2, and it carries the CAS Registry Number 526-94-3. This compound appears as a yellowish-white crystalline powder that is slightly soluble in water but dissolves readily in alkaline solutions containing oxidizing agents. The defining characteristic of 3-aminophthalhydrazide is its remarkable chemiluminescent property. When mixed with an oxidant, such as hydrogen peroxide, in the presence of a catalyst like iron ions or hemoglobin, it undergoes oxidation to form an unstable intermediate that decomposes into an excited state of 3-aminophthalate. As this molecule relaxes to its ground state, it emits a distinct blue glow, typically peaking around 425 nanometers.
The primary application of this substance lies in the detection of trace blood at crime scenes. Even minute quantities of blood, often invisible to the naked eye after cleaning, contain hemoglobin which acts as a potent catalyst for the luminol reaction. This allows investigators to visualize latent bloodstains over large areas, aiding significantly in criminal investigations. Beyond forensics, it serves as a sensitive probe for detecting other metal ions, peroxides, and specific enzymes in biological samples. In clinical diagnostics, modified versions are employed in immunoassays to quantify antigens or antibodies with high sensitivity. Furthermore, researchers utilize it to study oxidative stress and free radical generation within cellular systems due to its rapid response to reactive oxygen species. Despite its utility, handling requires caution as it can be irritating to the skin and eyes. Its ability to provide visual evidence through light emission without external illumination makes 3-aminophthalhydrazide an indispensable tool across multiple scientific disciplines, bridging the gap between microscopic chemical events and macroscopic observation.