*The following content is generated by AI and is for reference only.
<h3>Overview</h3>
<p><strong>(1-Oxyl-2,2,5,5-tetramethyl-∆3-pyrroline-3-methyl) Methanethiosulfonate</strong> is a specialized paramagnetic reagent used for site-specific protein labeling. Its core synthesis involves the coupling of stable nitroxide radicals with thiosulfonate groups to ensure high stability and specificity.</p>
<h3>Application</h3>
<ul>
<li><strong>Proteomics:</strong> Site-directed spin labeling (SDSL) for structural analysis via EPR spectroscopy.</li>
<li><strong>Drug Discovery:</strong> Monitoring conformational changes in membrane proteins during ligand binding assays.</li>
<li><strong>Bioconjugation:</strong> Labeling cysteine residues on antibodies or enzymes without disrupting biological activity.</li>
</ul>
<h3>Precautions</h3>
<p>Store under inert atmosphere at -20°C to prevent radical degradation. Avoid direct skin contact; use appropriate PPE as it may cause irritation. Not intended for clinical administration in humans.</p>