Introduction: Three (2-carboxyethyl) phosphine hydrochloride (TCEP hydrochloride) is a novel reducing and deprotection agent for thiolated DNA. The thiolated end sulfur atoms of DNA tend to form dimers in solution, especially in the presence of oxygen. This dimerization greatly reduces the efficiency of some coupling reaction experiments, such as DNA fixation in biosensors; Adding tris (2-carboxyethyl) phosphine hydrochloride (TCEP hydrochloride) to the DNA solution and removing it after a period of reaction can reduce DNA dimerization. Application Three (2-carbonylethyl) phosphate hydrochloride is an organic phosphorus compound that can be used as a water-soluble reagent for selective reduction of disulfides; More stable than DTChemicalbookT in mass spectrometry applications; It can also be used for selective reduction of disulfides in water. Bioactive TCEPhydrochloride (Tris (2-carboxyethyl) phosphinehydrochloride) is a non thiol reducing agent that is more stable and produces faster S-S reduction reactions than other chemical reducing agents. TCEPhydrochloride is a trialkylphosphine that selectively reduces protein degradation without altering its properties or interacting with thiol directing agents in the reaction mixture. TCEPhydrochloride is still a commonly used reducing agent in DNA/AuNP chemistry.
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