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<h3>Overview</h3>
<p><strong>5-Carboxyfluorescein</strong> (CAS 76823-03-5) is a highly fluorescent derivative of fluorescein used primarily as a tracer. Its molecular formula is C₂₀H₁₂O₅, synthesized via carboxylation of fluorescein.</p>
<h3>Application</h3>
<ul>
<li>Cell biology for tracking cell proliferation and migration.</li>
<li>DNA sequencing as a fluorescent labeling agent.</li>
<li>Flow cytometry analysis for intracellular marker detection.</li>
</ul>
<h3>Precautions</h3>
<p>Store in a cool, dry place away from light. Avoid direct contact with skin and eyes; use appropriate PPE during handling to prevent irritation.</p>