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<h3>Overview</h3>
<p>Bromophenol blue is a synthetic sulfonephthalein dye used primarily as an acid-base indicator and tracking dye in electrophoresis. It features a <strong>molecular formula</strong> of C<sub>19</sub>H<sub>10</sub>Br<sub>4</sub>O<sub>5</sub>S and <strong>CAS No.</strong> 115-39-9. Production involves the condensation of phenolphthalein with bromine under controlled acidic conditions to ensure high purity grades for laboratory and industrial use.</p>
<h3>Application</h3>
<ul>
<li>Analytical Chemistry: pH indicator ranging from 3.0 (yellow) to 4.6 (blue).</li>
<li>Molecular Biology: Tracking dye in SDS-PAGE and agarose gel electrophoresis.</li>
<li>Pharmaceutical Analysis: Used in titration methods for quality control testing.</li>
</ul>
<h3>Precautions</h3>
<p>Store in a cool, dry place away from light and incompatible oxidizers. Avoid direct contact with skin and eyes; wear appropriate PPE including gloves and goggles. Not intended for human consumption or clinical therapeutic use.</p>