Product Description
BisTrischloride;BIS-TRIS-HCL;2-(Bis(2-hydroxyethyl)aMino)-2-(hydroxyMethyl)propane-1,3-diolhydrochloride;EZ-96KITBACTERIALDNAMAG-BIND(4X96);EZ-96KITBACTERIALDNAMAChemicalbookG-BIND(1X96);BIS(2-HYDROXYETHYL)AMINOTRIS(HYDROXYMETHYL)METHANEHYDROCHLORIDE;BIS[2-HYDROXYETHYL]IMINOTRIS[HYDROXYMETHYL]METHANEHYDROCHLORIDE;BIS-TRISHYDROCHLORIDE
AI Product Description
*The following content is generated by AI and is for reference only.
Bis-Tris Hydrochloride, chemically designated as 2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol hydrochloride, is a critical buffering agent widely utilized in biochemical and molecular biology research. With the CAS number 64431-50-9 and the molecular formula C8H19NO5·HCl, this compound serves as the protonated form of Bis-Tris, offering superior stability across a specific pH range essential for sensitive biological assays. Its primary function lies in maintaining optimal physiological conditions during protein purification, electrophoresis, and enzyme kinetics studies.
The compound features a pKa value near 6.46 at 25°C, making it particularly effective for experiments requiring a slightly acidic to neutral environment. Unlike Tris buffers, Bis-Tris offers reduced interaction with metal ions and lower susceptibility to oxidation, which minimizes artifacts in enzymatic reactions and preserves protein integrity. Consequently, it is the buffer of choice for SDS-PAGE gels, where consistent pH control ensures sharp band resolution and accurate molecular weight determination. Furthermore, its low UV absorbance makes it ideal for spectrophotometric analyses and chromatography applications, such as ion-exchange and size-exclusion chromatography.
In pharmaceutical development, Bis-Tris Hydrochloride is employed to stabilize labile proteins and vaccines, preventing denaturation during storage and formulation. Its high solubility in water allows for easy preparation of stock solutions without precipitation issues. The hydrochloride salt form ensures immediate availability of protons, facilitating rapid pH adjustment without the need for additional acid titration. Researchers frequently utilize this reagent in cell culture media additives and DNA/RNA extraction protocols due to its compatibility with nucleic acids and minimal interference with downstream sequencing or amplification steps. As a standard component in analytical chemistry, it supports reproducible results in quality control laboratories. Overall, Bis-Tris Hydrochloride represents an indispensable tool for scientists seeking precise pH control, high chemical stability, and minimal background interference in complex biological systems, thereby driving advancements in proteomics, genomics, and drug discovery sectors globally.
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