*The following content is generated by AI and is for reference only.
<h3>Overview</h3>
<p><strong>6-FAM-Phosphoramidite</strong> is a fluorescently labeled nucleotide building block used in automated DNA synthesis. It features the 6-carboxyfluorescein (FAM) dye attached to the C6 position of deoxyuridine, enabling real-time detection via fluorescence. Molecular formula: C<sub>28</sub>H<sub>30</sub>N<sub>4</sub>O<sub>9</sub>P; CAS No.: 157857-56-4.</p>
<h3>Application</h3>
<ul>
<li><strong>Molecular Diagnostics:</strong> Designing TaqMan probes for qPCR pathogen detection.</li>
<li><strong>Gene Expression Profiling:</strong> Synthesizing microarray probes for high-throughput screening.</li>
<li><strong>SNP Genotyping:</strong> Creating allele-specific oligonucleotides for genetic variation analysis.</li>
</ul>
<h3>Precautions</h3>
<p>Store strictly under inert atmosphere at -20°C to prevent hydrolysis. Handle with appropriate PPE as phosphoramidites are moisture-sensitive and potential sensitizers. Not intended for direct clinical administration without further purification and formulation.</p>