Determination of Theanine by UV
1) Reagents
a.Phosphoric acid b. Acetronitrile(HPLC) c. Nanopure water d.Sodium acetate,trihydrate
e.EDTA f.NaN3 g.TEA h.Acetone i.ACQ-Tag reagent
2) Apparatus
HPLC (SHIMADZU) Analytical balance (1/10000) Ultrasonic cleaner
0.45μm PTFE filter 50ml volumetric flask Screw cap vials
Vortex mixer Oven capable of maintaining 110+1℃
3) HPLC condition
Column: Hypersil C18 5 mm 4.6′200mm
Detector: UV248nm
Mobile Phase:A: (1) Dilute Phosphoric acid 200ml :Add 100ml concentrated Phosphoric acid to 100ml waret
(2)EDTA solution: weigh 100mg EDTA ,add 100ml Milli-Q water sonicate to dissolve
(3)Weigh 19.04g sodium acetate ,add 1liter water ,stir bring pH to 5.2 with dilute phosphoric acid ,add 1ml EDTA solution ,0.1g NaN3,2.37ml TEA, titrate to pH4.95 with dilute phosphoric acid ,filter through aqueous solvent filter<...










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