What is custom peptide synthesis?
Custom peptide synthesis generally refers to a service of artificial peptide synthesis. It refers to the service of processing and synthesis to meet specific needs according to the needs of customers, such as different requirements of sequence, purity, molecular weight, etc.The molecular weight was confirmed by mass spectrometer to determine whether the crude MS was correct or not, and then the crude MS was purified by high performance liquid chromatography (HPLC) to obtain the quality peptide.According to different experiments, different purities of peptides can be selected. In principle, the higher the purity, the higher the price.
Gotop Biotech supplies various modified peptides according to customer requirements.
1.Phosphorylated Ser, Tyr, and Thr-modified peptides: We provide services for monophosphorylated and polyphosphorylated peptides, and we are currently able to provide four phosphorylation site-modified peptides.
2.5(6)-FAM, FITC, CY5, RhodamineB, PNA, EDNAS/dabcyl and other fluorescent-labeled modified peptides: Fluorescent-labeled modified peptide technology is our representative peptide synthesis technology of Gutuo Biology, and our technology has been quite mature.
3. Biotin, Lys (Biotin) modified peptides: Biotin is a component of vitamin B2, and Biotin, Lys (Biotin) modified peptides are also frequently customized by customers.We offer biotin-modified peptides with nearly 100% success rates.
4. Peptides containing one or more pairs of disulfide bonds: disulfide bonds play an important role in the structural stabilization of proteins, and we are currently able to provide customers with four pairs of disulfide bond-modified peptides.
5. Peptides Containing Isotope C13, N15 Modifications: Isotope-labeled peptides are mainly used in medical and biological fields, usually at higher prices. To meet customer needs, we accept microgram-scale customization of isotope peptides.
6. Peptides containing special amino acid modifications: e.g., D-type amino acids, amino acid derivatives, aliphatic carboxylic acids, etc., are all within the customized range we accept.
Gotop Biotech provides a long peptide synthesis service of less than 150 amino acids.
In the process of peptide synthesis, when the peptide chain is too long, there will often be residues, amino acid condensation difficulties and so on. Based on these phenomena, we have developed three effective ways to improve the success rate of the reaction:
1. Microwave synthesis method: For some amino acids which are difficult to condense in the synthesis process, we use the microwave method to synthesize, which has significant effect and greatly shortens the reaction time.
2. Fragment synthesis: When some peptides are difficult to synthesize by conventional synthesis methods, we will also condensate a few amino acids in a certain segment of the peptide as a whole onto the peptide chain. This method can also solve many problems in synthesis.
3. Hydrazide synthesis method: The method of synthesizing peptide by hydrazide method is to synthesize N-terminal Cys peptide andChemical selective reaction between C-terminal peptide hydrazides forms amide bonds to achieve peptide linkage. According to the position of Cys in the peptide chain, the whole peptide chain is divided into several sequences and synthesized separately. Finally, the target peptide is obtained by liquid phase condensation reaction, which significantly improves the purity of the final product and is widely applicable to the ...
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