*The following content is generated by AI and is for reference only.
<h3>Overview</h3>
<p>N4-tac-DMT-dC-CE-Phosphoramidite is a specialized <strong>phosphoramidite monomer</strong> designed for automated <strong>solid-phase oligonucleotide synthesis</strong>. Its structure incorporates a dimethoxytrityl (DMT) protecting group, a triazole-based linker (tac), and a cyanoethyl (CE) phosphite moiety attached to N4-acetyl-deoxycytidine. This reagent enables the efficient incorporation of modified cytosine residues into DNA strands.</p>
<h3>Application</h3>
<ul>
<li><strong>Antisense Therapy</strong>: Synthesis of gapmer sequences targeting specific mRNA transcripts.</li>
<li><strong>Diagnostic Probes</strong>: Manufacturing fluorescently labeled hybridization probes for qPCR.</li>
<li><strong>CRISPR Components</strong>: Production of guide RNAs requiring precise chemical modifications.</li>
</ul>
<h3>Precautions</h3>
<p>Store under inert atmosphere at -20°C to prevent hydrolysis. Handle in a fume hood due to potential toxicity of phosphoramidites. Strictly exclude moisture during synthesis to maintain coupling efficiency.</p>