*The following content is generated by AI and is for reference only.
<h3>Overview</h3>
<p><strong>Methotrexate EP Impurity D</strong> is a critical reference standard for the pharmaceutical analysis of methotrexate, defined by the European Pharmacopoeia. It serves as an identification and quantification tool in quality control laboratories to ensure drug purity during synthesis.</p>
<p><strong>Chemical Formula:</strong> C<sub>20</sub>H<sub>22</sub>N<sub>4</sub>O<sub>5</sub> | <strong>CAS No:</strong> 61795-28-2</p>
<h3>Application</h3>
<ul>
<li><strong>Quality Control:</strong> Used in HPLC assays to identify and quantify specific degradation products in methotrexate bulk drugs.</li>
<li><strong>Method Validation:</strong> Serves as a primary standard to validate analytical procedures for regulatory compliance.</li>
<li><strong>R&D:</strong> Supports stability studies to monitor impurity profiles under various stress conditions.</li>
</ul>
<h3>Precautions</h3>
<p>Store at -20°C in airtight containers protected from light and moisture. Handle with appropriate PPE due to potential cytotoxicity; avoid direct skin contact and inhalation of dust.</p>