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<h3>Overview</h3>
<p>Diethyl pyrocarbonate (DEPC) is a potent <strong>carbodiimide reagent</strong> used primarily for <strong>nucleic acid decontamination</strong>. Its molecular formula is C<sub>5</sub>H<sub>10</sub>O<sub>3</sub> with CAS No. 646-39-7. The compound functions by ethylating nucleophilic groups, effectively inactivating RNases.</p>
<h3>Application</h3>
<ul>
<li><strong>Molecular Biology:</strong> Sterilization of water and glassware for RNA work.</li>
<li><strong>Pharmaceuticals:</strong> Synthesis intermediate for drug manufacturing.</li>
<li><strong>Chemical Research:</strong> Protecting group introduction in organic synthesis.</li>
</ul>
<h3>Precautions</h3>
<p>Strictly avoid contact with skin and eyes; use in well-ventilated fume hoods. Store in a cool, dry place away from moisture to prevent hydrolysis into ethanol and CO<sub>2</sub>. Not approved for direct clinical administration due to toxicity.</p>