Isopropyl β-D-1-Thiogalactopyranoside
product description:
ISOPROPYL THIOGALACTOSIDE / IPTG
Isopropyl-thio -beta-D- galactopyranoside
CAS: 367-93-1
Chinese Name: isopropyl thio -β-D- galactopyranoside
Product name: Isopropyl β-D-1-Thiogalactopyranoside
English abbreviation: IPTG
Synonyms: Isopropyl β-D-Thiogalactoside
General CAS: 367-93-1
Product purity: Sigma ≥99%, Merck ≥98%, INALCO≥99%.
As β-galactosidase activity was induced biological activity identification.
Analysis to determine the purity by TLC.
Properties: white or white powder, soluble in water was clear and colorless liquid
Molecular formula: C9H18O5S
Molecular Weight: 238.30
Molecular Structure:
Storage temperature: 2-8 ° C cold storage, after a good preparation stored at -20 ° C, room temperature for one month
Uses: IPTG is β- galactosidase activity inducing substance. Based on this feature, when the pUC series vector DNA (or other vector with a lacZ gene DNA) deletion in lacZ host cells were transformed with the vector during DNA M13 phage were transfected or, if X is added to the plate medium -gal and of IPTG, since the β- galactosidase α- complementarity, rendering based on whether white colonies (or plaques) are conveniently selected recombinants. In addition, it can be used as the like having a lac or tac promoter expression vector expression inducer.