
ChromaX®A20 is a protein A affinity chromatography column designed to quickly and accurately determine the antibody concentration (or titer) in cell culture super-natant, which is developed by unique surface treatment technique and coupling technique using recombinant protein A as ligand and polystyrene divinylbenzene (PS-DVB) microspheres with a porous structure as filling carrier.
Hydrophilic PS-DVB substrate, with the features of good rigidity, low back pres- sure, and fast mass transfer.
Unique hydrophilic treatment technology with low non-specific adsorption en- sures the accuracy of antibody concentration test results.
Ligand specific binding sites, high selectivity ensures the accuracy of concentra- tion determination results.
Stainless steel column for direct use on HPLC.
Fast and efficient, taking only 2 - 5 minutes to complete an analysis.
Good linearity within the wide range of antibody concentration of 0.02 to 10 mg/mL
Adopting alkali-tolerant protein A ligand allows for regenerating the chromato- graphic column with the 0.1 M NaOH solution.
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