Determination of Asiaticoside and Madecasoside

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Determination of Asiaticoside and Madecasoside

February 29, 2024

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Determination of Asiaticoside and Madecasoside

1) Reagents

a. Nanopure water b. Acetonitrile (HPLC)

2) Apparatus

HPLC (Shimadzu) Analytical balance (1/10000) Ultrasonic cleaner

0.45μm PTFE filter 50ml volumetric flask Screw cap vials detector(Alltech)

3) HPLC condition

Column: Kromasil C18 5 mm 4.6′200mm

Mobile Phase: A:Water B: Acetonitrile

A:B=67:33

Flow Rate: 0.7ml/min

Injection: 10μl

Drift tube temperature :100.2℃

Gas flow:2.7L/min

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